Experiment Overview
| Repository ID: | FR-FCM-Z3WR | Experiment name: | human PBMC in COVID-19 | MIFlowCyt score: | 83.64% |
| Primary researcher: | Chen Yu | PI/manager: | Chen Yu | Uploaded by: | Chen Yu |
| Experiment dates: | 2020-05-01 - 2020-12-31 | Dataset uploaded: | Mar 2021 | Last updated: | Mar 2021 |
| Keywords: | [PBMC] [MAIT cell] [COVID-19] [SARS-CoV2] | Manuscripts: | |||
| Organizations: |
Duke University, Durham, (USA)
|
||||
| Purpose: | Sexual dimorphisms in immune responses contribute to coronavirus disease 2019 (COVID-19) outcomes, yet the mechanisms governing this disparity remain incompletely understood. We carried out sex-balanced sampling of peripheral blood mononuclear cells from confirmed COVID-19 inpatients and outpatients, uninfected close contacts, and healthy controls for 36-color spectral flow cytometry. | ||||
| Conclusion: | Our results revealed a pronounced reduction of circulating mucosal associated invariant T (MAIT) cells in infected females. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Single stained cells/beads | ||||
Experiment variables
| Conditions | |
|---|---|
| · healthy | 062CD8.fcs · 22CBD6.fcs · 52BA23.fcs · 655A91.fcs · 6FD678.fcs · 77DA77.fcs · 94D44E.fcs · B29A26.fcs · B79102.fcs · F5E7EF.fcs |
| · exposed | 5ABDCB_0.fcs · 5ABDCB_14.fcs · 5ABDCB_28.fcs · 61BBAD_0.fcs · 61BBAD_14.fcs · 61BBAD_7.fcs · 6F894A_0.fcs · 6F894A_14.fcs · 6F894A_7.fcs · 7768E4_14.fcs · 7768E4_2.fcs · 7768E4_28.fcs · 80A16A_0.fcs · 80A16A_14.fcs · B96D3B_0.fcs · B96D3B_14.fcs · B96D3B_28.fcs · CE0CE8_0.fcs · CE0CE8_14.fcs · CE0CE8_28.fcs |
| · infected | 0B943B_0.fcs · 0B943B_14.fcs · 0B943B_3.fcs · 0BF51C_0.fcs · 0BF51C_14.fcs · 0BF51C_7.fcs · 0E1F8E_0.fcs · 0E1F8E_14.fcs · 0E1F8E_7.fcs · 180E1A_0.fcs · 180E1A_14.fcs · 180E1A_7.fcs · 1A9B20_0.fcs · 1A9B20_14.fcs · 1A9B20_7.fcs · 24305F_1.fcs · 2AD75E_14.fcs · 2AD75E_7.fcs · 3F05F3_0.fcs · 3F05F3_28.fcs · 3F05F3_7.fcs · 40067F_0.fcs · 40067F_14.fcs · 40067F_21.fcs · 450905_14.fcs · 450905_7.fcs · 6AA2F7_0.fcs · 7085CA_0.fcs · 7085CA_14.fcs · 7085CA_7.fcs · 75A2B6_0.fcs · 75A2B6_14.fcs · 75A2B6_7.fcs · 82CCF5_0.fcs · 82CCF5_14.fcs · 82CCF5_7.fcs · 997B57_0.fcs · B85D75_0.fcs · B85D75_7.fcs · BAAF62_7.fcs · DF309F_0.fcs · DF309F_14.fcs · DF309F_7.fcs · F7F4D2_0.fcs |
| · hospitalized | 0993F8.fcs · 2DB5F9.fcs · 36EA16.fcs · 45FBA5.fcs · 460F8D.fcs · 936128.fcs · B3E924.fcs · EB057C.fcs · FE84A0.fcs |
| Individuals | |
|---|---|
| · female | 0BF51C_0.fcs · 0BF51C_14.fcs · 0BF51C_7.fcs · 180E1A_0.fcs · 180E1A_14.fcs · 180E1A_7.fcs · 1A9B20_0.fcs · 1A9B20_14.fcs · 1A9B20_7.fcs · 22CBD6.fcs · 2DB5F9.fcs · 36EA16.fcs · 6F894A_0.fcs · 6F894A_14.fcs · 6F894A_7.fcs · 6FD678.fcs · 75A2B6_0.fcs · 75A2B6_14.fcs · 75A2B6_7.fcs · 7768E4_14.fcs · 7768E4_2.fcs · 7768E4_28.fcs · 77DA77.fcs · 82CCF5_0.fcs · 82CCF5_14.fcs · 82CCF5_7.fcs · 94D44E.fcs · 997B57_0.fcs · B3E924.fcs · B85D75_0.fcs · B85D75_7.fcs · CE0CE8_0.fcs · CE0CE8_14.fcs · CE0CE8_28.fcs · DF309F_0.fcs · DF309F_14.fcs · DF309F_7.fcs · F5E7EF.fcs · FE84A0.fcs |
| · male | 062CD8.fcs · 0993F8.fcs · 0B943B_0.fcs · 0B943B_14.fcs · 0B943B_3.fcs · 0E1F8E_0.fcs · 0E1F8E_14.fcs · 0E1F8E_7.fcs · 24305F_1.fcs · 2AD75E_14.fcs · 2AD75E_7.fcs · 3F05F3_0.fcs · 3F05F3_28.fcs · 3F05F3_7.fcs · 40067F_0.fcs · 40067F_14.fcs · 40067F_21.fcs · 450905_14.fcs · 450905_7.fcs · 45FBA5.fcs · 460F8D.fcs · 52BA23.fcs · 5ABDCB_0.fcs · 5ABDCB_14.fcs · 5ABDCB_28.fcs · 61BBAD_0.fcs · 61BBAD_14.fcs · 61BBAD_7.fcs · 655A91.fcs · 6AA2F7_0.fcs · 7085CA_0.fcs · 7085CA_14.fcs · 7085CA_7.fcs · 80A16A_0.fcs · 80A16A_14.fcs · 936128.fcs · B29A26.fcs · B79102.fcs · B96D3B_0.fcs · B96D3B_14.fcs · B96D3B_28.fcs · BAAF62_7.fcs · EB057C.fcs · F7F4D2_0.fcs |
| Timepoints | |
|---|---|
| · early (<= 14 days) | 0993F8.fcs · 0B943B_0.fcs · 0B943B_3.fcs · 0BF51C_0.fcs · 0BF51C_7.fcs · 0E1F8E_0.fcs · 0E1F8E_7.fcs · 180E1A_0.fcs · 24305F_1.fcs · 36EA16.fcs · 3F05F3_0.fcs · 45FBA5.fcs · 460F8D.fcs · 5ABDCB_0.fcs · 6F894A_0.fcs · 6F894A_7.fcs · 7085CA_0.fcs · 7085CA_7.fcs · 7768E4_2.fcs · 82CCF5_0.fcs · 936128.fcs · B85D75_0.fcs · CE0CE8_0.fcs · DF309F_0.fcs · EB057C.fcs · F7F4D2_0.fcs · FE84A0.fcs |
| · middle (<= 21 days) | 0B943B_14.fcs · 0BF51C_14.fcs · 180E1A_7.fcs · 1A9B20_0.fcs · 2AD75E_7.fcs · 2DB5F9.fcs · 3F05F3_7.fcs · 40067F_0.fcs · 450905_7.fcs · 6F894A_14.fcs · 7085CA_14.fcs · 75A2B6_0.fcs · 75A2B6_7.fcs · 82CCF5_7.fcs · B85D75_7.fcs · BAAF62_7.fcs · DF309F_7.fcs |
| · late (> 21 days) | 0E1F8E_14.fcs · 180E1A_14.fcs · 1A9B20_14.fcs · 1A9B20_7.fcs · 2AD75E_14.fcs · 3F05F3_28.fcs · 40067F_14.fcs · 40067F_21.fcs · 450905_14.fcs · 5ABDCB_14.fcs · 5ABDCB_28.fcs · 61BBAD_0.fcs · 61BBAD_14.fcs · 61BBAD_7.fcs · 75A2B6_14.fcs · 7768E4_14.fcs · 7768E4_28.fcs · 80A16A_0.fcs · 80A16A_14.fcs · 82CCF5_14.fcs · 997B57_0.fcs · B3E924.fcs · B96D3B_0.fcs · B96D3B_14.fcs · B96D3B_28.fcs · CE0CE8_14.fcs · CE0CE8_28.fcs · DF309F_14.fcs |
| · healthy control | 062CD8.fcs · 22CBD6.fcs · 52BA23.fcs · 655A91.fcs · 6AA2F7_0.fcs · 6FD678.fcs · 77DA77.fcs · 94D44E.fcs · B29A26.fcs · B79102.fcs · F5E7EF.fcs |
