Experiment Overview

Repository ID: FR-FCM-Z6UG Experiment name: Determining the role of CD4+ and CD8+ T-cells in the LST response MIFlowCyt score: 100.00%
Primary researcher: Jalal Alshaweesh PI/manager: Shinjiro Hamano Uploaded by: Jalal Alshaweesh
Experiment dates: 2019-04-01 - 2023-10-01 Dataset uploaded: Oct 2023 Last updated: Oct 2023
Keywords: [CD3+CD4+] [CD3+CD8+] [lymphodepletion] [Leishmanin] [LST] Manuscripts:
Organizations: Nagasaki University, Department of Parasitology, Nagasaki , Nagasaki (Japan)
Purpose: Validate the Leishmania donovani LST leishmanin antigen in immune animals.
Conclusion: The cell depletion was confirmed in sampled mice using flow cytometry and Rag2-/- mice were used as a control for the depletion of CD4+ and CD8+ T-cells.
Comments: None
Funding: The Global Health Innovative Technology Fund (GHIT)
Quality control: Experiment included: Negative control, Positive control and FMO controls

Experiment variables

· FMO control Spleenocytes_Tcells_FMO- no CD4 staining_002.fcs
· Unstained Spleenocytes_Tcells_Unstained_control_001.fcs
· Undepleted Spleenocytes_Tcells_Vaccinated_Saline_004.fcs
· Rag2 KO control Spleenocytes_Tcells_Rag2KO_005.fcs
· Samples Spleenocytes_Tcells_Vaccinated_GK15_006.fcs · Spleenocytes_Tcells_Vaccinated_GK15__2_007.fcs · Spleenocytes_Tcells_Vaccinated_GK15__3_009.fcs · Spleenocytes_Tcells_Vaccinated_aCD8_003.fcs

Sample Type
· Spleenocytes Spleenocytes_Tcells_FMO- no CD4 staining_002.fcs · Spleenocytes_Tcells_Rag2KO_005.fcs · Spleenocytes_Tcells_Unstained_control_001.fcs · Spleenocytes_Tcells_Vaccinated_GK15_006.fcs · Spleenocytes_Tcells_Vaccinated_GK15__2_007.fcs · Spleenocytes_Tcells_Vaccinated_GK15__3_009.fcs · Spleenocytes_Tcells_Vaccinated_Saline_004.fcs · Spleenocytes_Tcells_Vaccinated_aCD8_003.fcs

Download FCS Files or login and see the dataset in your inbox for further annotation details.