Experiment Overview
| Repository ID: | FR-FCM-Z78Y | Experiment name: | OMIP_AML | MIFlowCyt score: | 65.50% |
| Primary researcher: | Jan Musil | PI/manager: | Jan Musil | Uploaded by: | Jan Musil |
| Experiment dates: | 2021-05-05 - 2022-06-30 | Dataset uploaded: | Mar 2024 | Last updated: | Mar 2024 |
| Keywords: | [NK cells] [T cells] [AML] [immunotherapy] [bone marrow] [high-dimensional flow cytometry] [Leukemic stem cells] [immune checkpoint] [inhibitory ligands] [checkpoint inhibitors] | Manuscripts: | [39192598] |
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| Organizations: |
Institute of Hematology and Blood Transfusion, Immunomonitoring and Flow Cytometry, Prague, (Czech Republic)
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| Purpose: | A 30-color panel for the analysis of check-point inhibitory networks in the bone marrow of acute myeloid leukemia patients | ||||
| Conclusion: | We have successfully developed a panel that can resolve T cells, Nk cells, myeloblasts, and hematopoietic stem and progenitor cells in the human bone marrow and provide information on their phenotypic features with regard to immune evasion. | ||||
| Comments: | None | ||||
| Funding: | European Regional Development Fund and the state budget of the Czech Republic (project AIIHHP: CZ.02.1.01/0.0/0.0/16_025/0007428, OP RDE, MEYS). Supported by MH CZ - DRO (Institute of Hematology and Blood Transfusion – IHBT, IN 00023736) | ||||
| Quality control: | QC was performed using BD CST and Spherotech Rainbow Beads prior to each experiment, Time versus fluorescence parameter was used to monitor acquisition stability, Fluorescence minus one (FMO) controls were used to assess resolution of weakly expressed markers and for assessment of spillover spreading error issues and compensation artifacts using NxN plots, PBMCs from standard donor were used to monitor batch effects. | ||||
