Experiment Overview
| Repository ID: | FR-FCM-Z7CH | Experiment name: | OMIP-111: Immune-Profiling of T Helper 1 (Th1), Th2, and Th17 Signatures in Murine Splenocytes by Targeting Intracellular Cytokines | MIFlowCyt score: | 50.00% |
| Primary researcher: | Soumik Barman | PI/manager: | Soumik Barman | Uploaded by: | Soumik Barman |
| Experiment dates: | 2023-10-12 - 2024-12-23 | Dataset uploaded: | Apr 2024 | Last updated: | Mar 2025 |
| Keywords: | [spectral flow cytometry] [murine T cells] [Murine splenocytes] [Th1 cells] [Th2 cells] [Th17 cells] [Mitogen] [Sony ID7000] | Manuscripts: | [40095325] [40095325] |
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| Organizations: |
Boston Children's Hospital, Precision Vaccines Program, Boston, MA (USA)
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| Purpose: | Immune-profiling of T helper 1 or Th1 (IFNg, TNF and IL-2), Th2 (IL-4 and 5) and Th17 (IL-17) signatures in murine splenocytes by targeting intracellular cytokines using spectral flow cytometry. | ||||
| Conclusion: | Using spectral flow cytometry, we developed a 17-parameter panel to profile major immune cell subsets, memory phenotypes and functional cytokines (Th1/2/17) in murine splenocytes in steady state as well as in stimulated conditions. Our panel could be easily modified, if researchers wish to tailor the panel to their specific needs. | ||||
| Comments: | https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.24926 | ||||
| Funding: | David J. Dowling’s laboratory is funded in part by the U.S. National Institute of Health (NIH) / National Institutes of Allergy and Infectious Disease (NIAID) Vaccine Adjuvant Discovery Program Contracts #75N93019C00044 and #75N93024C00020. | ||||
| Quality control: | Quality control of the ID7000 is performed daily by the DFCI cytometry core using AlignCheck Particles (Sony, Cat# AE700510). | ||||
Experiment variables
| Sample Type | |
|---|---|
| · C57BL/6 | E02 C57_M1_BFA_TS WLSM.fcs · E03 C57_M2_BFA_TS WLSM.fcs · E04 C57_M3_BFA_TS WLSM.fcs · E05 C57_M4_BFA_TS WLSM.fcs · E06 C57_M5_BFA_TS WLSM.fcs · F02 C57_M1_Stim_TS WLSM.fcs · F03 C57_M2_Stim_TS WLSM.fcs · F04 C57_M3_Stim_TS WLSM.fcs · F05 C57_M4_Stim_TS WLSM.fcs · F06 C57_M5_Stim_TS WLSM.fcs |
| · BALB/c | E07 BALB_M1_BFA_TS WLSM.fcs · E08 BALB_M2_BFA_TS WLSM.fcs · E09 BALB_M3_BFA_TS WLSM.fcs · E10 BALB_M4_BFA_TS WLSM.fcs · E11 BALB_M5_BFA_TS WLSM.fcs · F07 BALB_M1_Stim_TS WLSM.fcs · F08 BALB_M2_Stim_TS WLSM.fcs · F09 BALB_M3_Stim_TS WLSM.fcs · F10 BALB_M4_Stim_TS WLSM.fcs · F11 BALB_M5_Stim_TS WLSM.fcs |
| Conditions | |
|---|---|
| · Unstimulated (BFA treated) | E02 C57_M1_BFA_TS WLSM.fcs · E03 C57_M2_BFA_TS WLSM.fcs · E04 C57_M3_BFA_TS WLSM.fcs · E05 C57_M4_BFA_TS WLSM.fcs · E06 C57_M5_BFA_TS WLSM.fcs · E07 BALB_M1_BFA_TS WLSM.fcs · E08 BALB_M2_BFA_TS WLSM.fcs · E09 BALB_M3_BFA_TS WLSM.fcs · E10 BALB_M4_BFA_TS WLSM.fcs · E11 BALB_M5_BFA_TS WLSM.fcs |
| · Stimulated (Mitogen treated) | F02 C57_M1_Stim_TS WLSM.fcs · F03 C57_M2_Stim_TS WLSM.fcs · F04 C57_M3_Stim_TS WLSM.fcs · F05 C57_M4_Stim_TS WLSM.fcs · F06 C57_M5_Stim_TS WLSM.fcs · F07 BALB_M1_Stim_TS WLSM.fcs · F08 BALB_M2_Stim_TS WLSM.fcs · F09 BALB_M3_Stim_TS WLSM.fcs · F10 BALB_M4_Stim_TS WLSM.fcs · F11 BALB_M5_Stim_TS WLSM.fcs |
