Experiment Overview
| Repository ID: | FR-FCM-Z8H9 | Experiment name: | A 24-Marker Flow Cytometry Panel for Comprehensive Immune Profiling Using Spectral Flow Cytometry | MIFlowCyt score: | 82.81% |
| Primary researcher: | Arun Arunachalam | PI/manager: | Arun Arunachalam | Uploaded by: | Arun Arunachalam |
| Experiment dates: | 2023-09-13 - 2024-05-22 | Dataset uploaded: | Nov 2024 | Last updated: | Jun 2025 |
| Keywords: | [Immunophenotyping] [human PBMCs] [immunotherapy] [spectral flow cytometry] [immune cells] | Manuscripts: | |||
| Organizations: |
Lerner Research Institute, Cleveland Clinic, Center for Immunotherapy and Precision Immuno-Oncology, Cleveland, OH (United States)
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| Purpose: | To comprehensively immunophenotype the common and rare peripheral blood mononuclear cell subsets in patients with solid and hematological malignancies treated with immunotherapy | ||||
| Conclusion: | This 24 marker panel enables reliable identification of myeloid and monocyte derived suppressor cells (MDSC), basophils, myeloid and plasmacytoid dendritic cells, classical, intermediate and non-classical monocytes, NK cell subsets, CD4 and CD8 conventional T cells, TCRαβ and TCRγδ T cells, Regulatory T cells, B cells and plasma cells from mononuclear cells. | ||||
| Comments: | None | ||||
| Funding: | None | ||||
| Quality control: | Daily instrument QC using SpectroFlo® QC beads prior to acquiring reference controls and samples to ensure optimal performance of the cytometer. Titration of all the markers using the same population, single stained reference controls using beads/cells for unmixing and generating compensation matrix, NxN plots using the controls to assess the accuracy of spill over correction and compensation. | ||||
