Experiment Overview
| Repository ID: | FR-FCM-ZYND | Experiment name: | Surface Marker Flow Cytometry in human eosinophils after glucocorticoid exposure | MIFlowCyt score: | 100.00% |
| Primary researcher: | Michelle Makiya | PI/manager: | Luis Franco | Uploaded by: | Michelle Makiya |
| Experiment dates: | 2017-12-11 - 2017-12-14 | Dataset uploaded: | Jul 2018 | Last updated: | Jul 2018 |
| Keywords: | [Eosinophils] [glucocorticoids] [CXCR4] [CCR1] [CCR3] | Manuscripts: | [29885264] |
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| Organizations: |
National Institute of Allergy and Infectious Diseases, Laboratory of Parasitic Diseases, Bethesda, MD (USA)
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| Purpose: | Verify transcriptional changes from methylprednisolone treatment. After in vitro culture of peripheral blood leukocytes +/- methylprednisolone determine changes in surface expression of CCR1, CCR3 and CXCR4. | ||||
| Conclusion: | CXCR4 expression is upregulated in eosinophils upon methylprednisolone treatment of peripheral blood leukocytes. | ||||
| Comments: | None | ||||
| Funding: | This research was supported by the Intramural Research Program of the National Institute of Allergy and Infectious Diseases (NIAID) at the National Institutes of Health. | ||||
| Quality control: | 6 Normal Donor samples were run with identical conditions, treatments and time points, single color controls of each fluorochrome were run for compensation and FMO controls were run for gating | ||||
Experiment variables
| Doses | |
|---|---|
| · 20 ug/dL Methylprednisolone | Patient_12_120min_MP20.fcs · Patient_13_120min_MP20.fcs · Patient_14_120min_MP20.fcs · Patient_15_120min_MP20.fcs · Patient_16_120min_MP20.fcs · Patient_17_120min_MP20.fcs |
| · 200 ug/dL Methylprednisolone | Patient_12_120min_MP200.fcs · Patient_13_120min_MP200.fcs · Patient_14_120min_MP200.fcs · Patient_15_120min_MP200.fcs · Patient_16_120min_MP200.fcs · Patient_17_120min_MP200.fcs |
| · Vehicle (0.1% ethanol) | Patient_12_120min_VH.fcs · Patient_13_120min_VH.fcs · Patient_14_120min_VH.fcs · Patient_15_120min_VH.fcs · Patient_16_120min_VH.fcs · Patient_17_120min_VH.fcs |
| · No treatment | Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs |
| Timepoints | |
|---|---|
| · Baseline | Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs |
| · 120 minute | Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs |
| Individuals | |
|---|---|
| · Patient_12 | Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_12_Baseline_None.fcs · Patient_12_Unstained.fcs |
| · Patient_13 | Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_13_Baseline_None.fcs · Patient_13_Unstained.fcs |
| · Patient_14 | Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_14_Baseline_None.fcs · Patient_14_Unstained.fcs |
| · Patient_15 | Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_15_Baseline_None.fcs · Patient_15_Unstained.fcs |
| · Patient_16 | Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_16_Baseline_None.fcs · Patient_16_Unstained.fcs |
| · Patient_17 | Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs · Patient_17_Baseline_None.fcs · Patient_17_Unstained.fcs |
| Conditions | |
|---|---|
| · Unstained | Patient_12_Unstained.fcs · Patient_13_Unstained.fcs · Patient_14_Unstained.fcs · Patient_15_Unstained.fcs · Patient_16_Unstained.fcs · Patient_17_Unstained.fcs |
| · Stained | Patient_12_120min_MP20.fcs · Patient_12_120min_MP200.fcs · Patient_12_120min_VH.fcs · Patient_12_Baseline_None.fcs · Patient_13_120min_MP20.fcs · Patient_13_120min_MP200.fcs · Patient_13_120min_VH.fcs · Patient_13_Baseline_None.fcs · Patient_14_120min_MP20.fcs · Patient_14_120min_MP200.fcs · Patient_14_120min_VH.fcs · Patient_14_Baseline_None.fcs · Patient_15_120min_MP20.fcs · Patient_15_120min_MP200.fcs · Patient_15_120min_VH.fcs · Patient_15_Baseline_None.fcs · Patient_16_120min_MP20.fcs · Patient_16_120min_MP200.fcs · Patient_16_120min_VH.fcs · Patient_16_Baseline_None.fcs · Patient_17_120min_MP20.fcs · Patient_17_120min_MP200.fcs · Patient_17_120min_VH.fcs · Patient_17_Baseline_None.fcs |
