Experiment Overview

Repository ID: FR-FCM-ZYQ9 Experiment name: Immune Subsets in Healthy Donor Bone Marrow MIFlowCyt score: 76.13%
Primary researcher: Meghali Goswami PI/manager: Meghali Goswami Uploaded by: Meghali Goswami
Experiment dates: 2017-02-09 - 2017-03-17 Dataset uploaded: Sep 2018 Last updated: Oct 2020
Keywords: [B cells] [flow cytometry] [NK cells] [monocytes] [healthy controls] [T cells] [dendritic cells] [bone marrow] [bone marrow mononuclear cells] Manuscripts: [30518681] [PMC6328018]
Organizations: National Heart, Lung, and Blood Institute (NHLBI), NIH, Hematology Branch, Bethesda, MD (USA)
Purpose: To perform a comprehensive assessment of immune cell subsets in bone marrow from a cohort of adult human healthy donors with a large age range.
Conclusion: This resource provides a reference dataset for immune cell populations in healthy human bone marrow across a wide age range. Single cell RNA-sequencing quantification of marrow-resident cell populations has good concordance with immunophenotyping by flow and mass cytometry with some discrepancies in T and NK subsets.
Funding: This work was supported by the Intramural Research Program of the National Heart, Lung, and Blood Institute of the National Institutes of Health.
Quality control: Daily quality assurance was performed using Cytometer setup and Tracking beads (BD) as per manufacturer’s recommendation along with 1peak rainbow Beads (BD) and 8 peak beads (Spherotec)(Perfetto et al., 2012; Wang and Hoffman, 2017).

Experiment variables

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