Experiment Overview
| Repository ID: | FR-FCM-ZZ3Z | Experiment name: | OMIP-016: Characterization of Antigen-Responsive macaque and human T-cells (Online Figure 6) | MIFlowCyt score: | 85.88% |
| Primary researcher: | Antonio Cosma | PI/manager: | Antonio Cosma | Uploaded by: | Antonio Cosma |
| Experiment dates: | 2009-09-28 - 2009-10-02 | Dataset uploaded: | Sep 2012 | Last updated: | Mar 2013 |
| Keywords: | [Innate Immune Response] [CD4 T cells; HIV; SIV; vaccines] [Intracellular cytokines; CD8 T cells; optimization] | Manuscripts: | [23184609] |
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| Organizations: |
CEA, Division of Immuno-Virology, DSV/iMETI, Fontenay-aux-Roses, (France)
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| Purpose: | To evaluate the staining intensity of the anti-TNF-a antibody clone MAb11 conjugated to different fluorochromes | ||||
| Conclusion: | The TNF-a Alexafluor 700 resulted to be the best antibody in the context of our panel | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Mock stimulation and SEB stimulation were compared. | ||||
Experiment variables
| Conditions | |
|---|---|
| · NS | CD3A700 5uL TNFa PC7_16322 NS_C01.fcs · CD3A700 5uL TNFaAPC_16322 NS_C02.fcs · CD3PC7 TNFaA700 2uL_16322 NS_C04.fcs |
| · SEB | CD3A700 5uL TNFa PC7_16322 SEB 4ug,2f,mL_D01.fcs · CD3A700 5uL TNFaAPC_16322 SEB 4ug,2f,mL_D02.fcs · CD3PC7 TNFaA700 2uL_16322 SEB 4ug,2f,mL_D04.fcs |
| Individuals | |
|---|---|
| · 16322 | CD3A700 5uL TNFa PC7_16322 NS_C01.fcs · CD3A700 5uL TNFa PC7_16322 SEB 4ug,2f,mL_D01.fcs · CD3A700 5uL TNFaAPC_16322 NS_C02.fcs · CD3A700 5uL TNFaAPC_16322 SEB 4ug,2f,mL_D02.fcs · CD3PC7 TNFaA700 2uL_16322 NS_C04.fcs · CD3PC7 TNFaA700 2uL_16322 SEB 4ug,2f,mL_D04.fcs |
