Experiment Overview
| Repository ID: | FR-FCM-ZZEB | Experiment name: | OMIP-024: Pan-leukocyte immunophenotypic characterization of human PBMC subsets (18-color panel) | MIFlowCyt score: | 52.46% |
| Primary researcher: | Gemma Moncunill | PI/manager: | Stephen DeRosa | Uploaded by: | Gemma Moncunill |
| Experiment dates: | 2014-04-23 - 2014-04-23 | Dataset uploaded: | Jul 2014 | Last updated: | Nov 2014 |
| Keywords: | [B cells] [NK cells] [monocytes] [Phenotyping] [human PBMC] [T cells] [γδ T cells] [activation] [differentiation] | Manuscripts: | [25352070] |
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| Organizations: |
Fred Hutchinson Cancer Research Center, Seattle, WA (USA)
Barcelona Centre for International Health Research (CRESIB), Barcelona, (Catalonia, Spain) |
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| Purpose: | Panel development for the measurement of relative frequencies of monocytes, B cells, plasmablasts, CD4+ and CD8+ T cells, regulatory T cells, gd T cells, NK T cells, NK cells, and dendritic cells in cryopreserved PBMC, including detection of activation and differentiation markers. | ||||
| Conclusion: | This panel allows the identification of monocytes, B cells, plasmablasts, CD4+ and CD8+ T cells, regulatory T cells, gd T cells, NK T cells, NK cells, and dendritic cells as well as the activation (HLA-DR and CD38) and the differentiation (CD45RA, CCR7, CD127, CD57) phenotype. Additionally, it includes the NKG2C marker. | ||||
| Comments: | None | ||||
| Funding: | Not disclosed | ||||
| Quality control: | Fluorescence Minus One tests | ||||
